Directed evolution to re-adapt a co-evolved network within an enzyme
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چکیده
منابع مشابه
Directed evolution to re-adapt a co-evolved network within an enzyme
We have previously used targeted active-site saturation mutagenesis to identify a number of transketolase single mutants that improved activity towards either glycolaldehyde (GA), or the non-natural substrate propionaldehyde (PA). Here, all attempts to recombine the singles into double mutants led to unexpected losses of specific activity towards both substrates. A typical trade-off occurred be...
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Applying artificial selection to create new proteins has allowed us to explore fundamental processes of molecular evolution. These "directed evolution" experiments have shown that proteins can readily adapt to new functions or environments via simple adaptive walks involving small numbers of mutations. With the entire "fossil record" available for detailed study, these experiments have provided...
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Enzymes that are adapted to widely different temperature niches are being used to investigate the molecular basis of protein stability and enzyme function. However, natural evolution is complex: random noise, historical accidents and ignorance of the selection pressures at work during adaptation all cloud comparative studies. Here, we review how adaptation in the laboratory by directed evolutio...
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An in vitro evolution procedure was used to obtain RNA enzymes with a particular catalytic function. A population of 10(13) variants of the Tetrahymena ribozyme, a group I ribozyme that catalyzes sequence-specific cleavage of RNA via a phosphoester transfer mechanism, was generated. This enzyme has a limited ability to cleave DNA under conditions of high temperature or high MgCl2 concentration,...
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ژورنال
عنوان ژورنال: Journal of Biotechnology
سال: 2012
ISSN: 0168-1656
DOI: 10.1016/j.jbiotec.2011.11.017